Mass Spectrometry Facility
Policies
Guidelines for using Open Access LC-MS
- Attend a training course provided by the mass spectrometry lab manager. (Must be completed before using the instrumentation.)
- For LC users, reserve the machine 24 hours in advance by emailing lab manager and signing the log book.
- Filter all samples with a 2 micron particle size filter prior to running.
- Use volatile solvents and avoid salts and buffers.
- Log all samples into the log book providing details listed therein.
- Do not overwrite others saved methods.
- Transfer data files from the instrument computer within 3 weeks. (Data files will be deleted without notice after 3 weeks.)
- If no one signs up for APCI, the source will remain ESI.
- After your analysis is complete, wash off the peak relevant to you several times with an appropriate solvent.
- If you happen to run out of Nitrogen during a run and change a cylinder, please inform lab manager.
- If Fused Silica Capillary or Ferrules are found choked or broken, please inform lab manager.
- Do not share the username and password for the LC MS computer with anyone else.
- Contact mass spectrometry lab personnel if any other problem is encountered.
Guidelines for using Open Access MALDI-TOF-MS
- Attend a training course provided by the mass spectrometry lab manager. (Must be completed before using the instrumentation.)
- Reserve the machine 24 hours in advance by emailing lab manager and signing the log book.
- Log all samples into the log book providing details listed therein.
- Zip tip samples.
- Do not overwrite saved method files.
- Do not use the MALDI computer to surf the net.
- Contact mass spectrometry lab personnel if any other problem is encountered.
If you are submitting samples for MALDI-TOF analysis, you are expected to provide the following details:
- Molecular Weight of protein
- Source of protein
- Enzyme used for digestion of protein
- Any modifications that may have been performed
- Approximate concentration of sample
IF YOU ARE DONE WITH ANALYSIS AFTER 5 PM, PLEASE TURN OFF THE LIGHTS AND LOCK THE DOOR.